Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYRLSMSLIANT-TVVRGRFLDIQQTVSEPTDIPNQVRYLEDGVLISEHGKIKWFGAWEDAQQHLPAGVEVQHYPEQLIVPGFIDTHIHFPQTEMVGAYG---EQLLSWLNTYTFP-----TEIQFQDKTYASEIAQFFVQELLKHGTTTALVFCTVHP-----ESVDALFEAAERVQMRLIAGKVLMDRNAPEA-L-----CDTPETAYSNTKALIEKWHG---KGRALYAITPRFAPTSTPEQLERAGQLKQEFPDVYVHTHLSENKDEIAWVKSLFPEQAGYLDVYQHYGLTGKRSVFAHCVHLEDEEWQCMHDTQSAIAFCPTSNLFLGSGLFPLKKTWEKQVKVGLGTDIGAGT-SFSLLQTVNEAYKVQQLQ-----GDKLSAYEALYHATLGGAKALDLQDQLGNFNIGKEADFVVLNLKPTALQELRQTKSKSVEDSLFALFTLGDDRNIEATYIYGNRAYQKETAK 5HMF Chain:A ((3-438)) -------MILIRGLTRVITF---------------DDQERELEDADILIDGPKIVAVGKNLSD----RSVSRTIDGRGMIALPGLINSHQHLYEGAMRAIPQLERVTMASWLEGVLTRSAGWWRDGKF-GPDVIREVARAVLLESLLGGITTVADQHPFFPGATADSYIDATIEAATDLGIRFHAVRSSMTLGKSEGGFCDDLFVEPVDRVVQHCLGLIDQYHEPEPFGMVRIALGPCGVTHDKPELFEAFAQMAADY-DVRLHTHFYQPLDAG-MSDHL--YGMTPWRFLEKHGWASDRVWLAHAVVPPREEIPEFADAGVAIAHLIAPDLRLGWGLAPIREYLDAGITVGFGTTGSASNDGGNLLGDLRLAALAHRPADPNEPEKWLSARELLRMATRGSAECLGRP-DLGVLEEGRAADIACWRLDGVDRV--------GVHDPAIGLIMTGLSDRASLVVVNGQVLVENERP-

General information: TITO was launched using: RESULT: Template: 5HMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2435 -41483 -17.04 -101.92 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -17.04 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.451

(partial model without unconserved sides chains): PDB file : Tito_5HMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HMF-query.scw PDB file : Tito_Scwrl_5HMF.pdb: