Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQPFVLYNSEQRKKVEFVPRKEGHIDMYVCGMTVYDYCHIGHARVMVAFDYIIRFLRSQGWKVRYIRNITDIDDKIIKRANENGETIQQLTMRFIDAMNEDAANLGCLAPDEAPKAT--EYIDQMQNMIGNLVNKGAAYPASNGDVYFEVTKFEKYGRLSGRKLDDMQAGASE----RV----------DVE-VE------------KKHP---FDFVLWKH-AKEN--EPSW---ASPWGNGRPGW--HIECSAMS--TCCL-------------GNHFDIHGGGSDLMFPHHENEIAQSEASTGEQYVNYWIHVGFINVD-GEKMSKSLGNFFTIRDVMEKFHPEVIRYFIVSS-HYRSPVNFSDVALKEAKT--SLTRFYHSFKAYQQVYGQTT-----------TETLDQSFVERFNNAMCDDFNTAEAMAVLFELNKELNRAVKEEQADQATVLYSTLRHLTNILGLVQHNVDDFLKSDIGQEALALSDAEIEDFIQQRVDAKKAKDFAKADSIRQSLLEQGVVLEDTRQGTVWRRAD 1A8H Chain:A ((4-423)) -----------------------VFYVTTPIYYVNAEPHLGHAYTTVVADFLARWHRLDGYRTFFLTGTDEHGETVYRAAQAAGEDPKAFVDRVSGRFKRAWDLLGIA-YDDFIRTTEERHKKVVQLVLKKVYEAGDIYYGEY-EGLYCVSCE-RF--YTEKELV---EGLCPIHGRPVERRKEGNYFFRMEKYRPWLQEYIQENPDLIRPEGYRNEVLAMLAEPIGDLSISRPKSRVPWGIPLPWDENHVTYVWFDALLNYVSALDYPEGEAYRTFWPHAWHLIGKDILKPHAVFWPTMLKAA-GIPMYRHLNVGGFLLGPDGRKMSKTLGNVVDPFALLEKYGRDALRYYLLREIPYGQDTPVSEEALRTRYEADLADDLGNLVQRTRAMLFRFAEGRIPEPVAGEELAEGTGLAGRLRPLVR-ELKFHVALEEAMAYVKALNRYINEKKP------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1A8H.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1779 -35327 -19.86 -100.93 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -19.86 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_1A8H.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1A8H-query.scw PDB file : Tito_Scwrl_1A8H.pdb: