Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNGMEEHLLTQKLQQRLRFSTHIQEAQAAVLIAITNENNPKVLLTRRSIHMNNHAGEVSFPGGKRDPSDTSNIVVALREAQEETALNPFDVQLLGDLPMQRARSGLSVKPIVGLIPPEVTLIPQPTEIDRIFFVPLQQLIEMRPTPYEVRYAHQSLYFPSLQIDNEII-WGLTAQ-MLIALFKYGLGYQKEWPFLLNAPTFGMPKFSHKK 2B0V Chain:A ((6-153)) --------------------------KPNVTVAAVIEQDDKYLLVEEI--PRGTAIKLNQPAGHLEPGES-IIQACSREVLEETGHSFLPEVLTG----------------------IYHWTCASNGTTYLRFTFSGQVVSFDPDRKLDTGIVRAAWFSIDEIRAKQAMHRTPLVMQCIEDYHAGKRYPLDILQYYDGS-----------

General information: TITO was launched using: RESULT: Template: 2B0V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 669 -39831 -59.54 -272.82 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -59.54 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.280

(partial model without unconserved sides chains): PDB file : Tito_2B0V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B0V-query.scw PDB file : Tito_Scwrl_2B0V.pdb: