Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSTPEQLPALSAAGSPSSGGASSHHSPHEASLSFPASVIPGDASAESPRAWSMPRSSTASEESAATSQLQQKPDAPESRLGGRKVVYFVDHSVTSIAYAEGHLMRPSRVRALHALVHSLGLDNAECMTVCHARPATAEEMGAFHRSAYLECLRQAPVICGNPLDEMSLAFQKEFDVPFASQNG--DCPLFPEVWALVSSQAGASLACAEALVRGDATVAMNWAGGMHHAAAAHASGFCFVNDIVLCIRRLLRHYQRVLYVDLDVHHGDGVEGAFYGNHRVMTLSLHQFGNGFFPGTGDYPTRETADS--FAINVPLPTRTGDAAYLLSFRTALSSVVQCFDPEAMVVQCGADTIAGDLIGRLCVTTLAHTQCVADVLSLERPTVLLGGGGYHVFHTARCWAIHTATALGRT-AAQLP---LYIPRTDPYYMDYRRECTPKRPTLHVFLDPDVDDPLPLGDSLAFWRQLCRSIQWQMRAARLVRQGFFRTLQLCRQRRAALLRQFATQEAGRESGIGVSGPKRPRSANATDRDAKEGVEDRVVSA 4RN1 Chain:B ((13-360)) -----------------------------------------------------------------------------------SLVPVYIYSPEYVSMCDSLAKIPKRADMVHSLIEAYALHKQ--MRIVKPKVASMEEMATFHTDAYLQHLQK-------------VSQ----DHPDSIEYGLG-CPATEGIFDYAAAIGGATITAAQCLIDGMCKVAINWSGGWHHAKKDEASGFCYLNDAVLGILRLRRKFERILYVDLDLHHGDGVEDAFSFTSKVMTVSLHKFSPGFFPGTGDVSDVGLGKGRYYSVNVPIQDGIQDEKYYQICESVLKEVYQAFNPKAVVLQLGADTIAGDPMCSFNMTPVGIGKCLKYILQWQLATLILGGGGYNLANTARCWTYLTGVILGKTLSSEIPDHEFFTAYGPDYVLEITPSCRPDRNEPH---------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4RN1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2040 -141231 -69.23 -422.85 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -69.23 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_4RN1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RN1-query.scw PDB file : Tito_Scwrl_4RN1.pdb: