Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MSKKR-----RNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLPEDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEM--------------REAVEKSEASSEEVPSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTE---EVEEEQNLSRLDQED-----SEKKSKKGFILTVLA----LVSVIIC---------VSAY-----YVYRQVARSTKEIETSQ----------STTANQSDVDDFNTLYDAFYTN--SNKTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIVD-GVL-----DTNAKAKSDAKFTDIKTGNTELDKVLDK--------------------------AISLGKSQQTSTSSSSSSQ--------TSSSSSSQASSNTTSEPKPSSSNE--------------TRSSRSEVNMGLSSA------GVAVQRSASRVAYNQSAI------------------DDSNNSAWDFA---DGVLEQI------LATSRSRG---YITGDQ----------------YILERVNIVNGNGYYNLYK---PDGTYLFTLNCKTGYFVGNGAGHADDLDY 5EFV Chain:A ((11-648)) GSMSNKLITDLSRVFDYRYVDENEYNFKLISDMLTDFNFSLEYHRNKEVFAHDGEQIKYEHLNVTSNVSDFLTYLNGRFSNMVLGHNGDGINEVKDARVDNTGYGHKTLQDRLYHDYSTLDVFTKKVEKAVDEHYKEYRATEYRFEPKEQEPEFITDLSPYTNAVMQSFWVDPRTKIIYMTQARPGNHYMLSRLKPNGQFIDRLLVKNGGHGTHNAYRYIDGELWIYSAVLDSNKNNKFVRFQYRTGEITYGNEMQDVMPNIFNDRYTSAIYNPVENLMIFRREYKPTERQLKNSLNFVEVRSADDIDKIDKVLYQMDIPMEYTSDTQPMQGITYDAGILYWYTGDSNTANPNYLQGFDIKTKELLFKRRIDIGGVNFQEAEGLDMYYDLETGRKALLIGVTIGPGNNRHHSIYSIGQRGVNQFLKNIAPQVSMTDSGGRVKPLPIQNPAYLSDITEVGHYYIYTQDTQNALDFPLPKAFRDAGWFLDVLPGHYNGALRQVLTRNSTGRNMLKFERVIDIFNKKNNGAWNFCPQNAGYWEHIPKSITKLSDLKIVGLDFYITTEESNRFTDFPKDFKGIAGWILEVKSNTPGNTTQVLRRNNFPSAHQFLVRNFGTGGVGKWSLFEGKVVE-

General information: TITO was launched using: RESULT: Template: 5EFV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1893 130206 68.78 281.22 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 68.78 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.135

(partial model without unconserved sides chains): PDB file : Tito_5EFV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EFV-query.scw PDB file : Tito_Scwrl_5EFV.pdb: