Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRLLVIGCGGVAQVAISKICQDSETFTEIM-IASRTKSKCDDLKAKLEGKTSTKIETAALDADKVEEVIALIGSYKPEAVLNVALPYQDLTIMDACLATGVHYIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSGFDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYWEDGKWVEVEAMSIKREYDFP-QVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSYLTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGVKD-GVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE-LDPDPFMEALNEYGLPWVVVENPQMVD 3BIO Chain:A ((9-299)) KIRAAIVGYGNIGRYALQALREAPD--FEIAGIVRR-------ELQP--------FRV-V---SD---IEQL-E--SVDVALVCSPSREVERTALEILKKGICTADSFDIHDG------------------------ILALRRSLGDAAGKSGAAAVIASGWDPGSDSVVRTLMQAI---VPKGITYTNFGPGM---SMGHTVAVKA-ID-------GVKAAL-----------SMTIPLGTGVHRRMVYVELLPGHNLEEVS--AAIK-ADEYFVHDETHV-----------------------IQVDE---VDAL----I-DMGHGVRMVRKGVSGSTQNQRMSFDM--EI---------N-NPALTGQVLVCAARAAMR--Q-QPGAYTLQEIPVIDLLPGDREQW--------------

General information: TITO was launched using: RESULT: Template: 3BIO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1576 -60202 -38.20 -215.01 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -38.20 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_3BIO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3BIO-query.scw PDB file : Tito_Scwrl_3BIO.pdb: