Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLIGYNTDGKGFFKCL--PSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEKTRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ 3SEF Chain:C ((33-294)) ----------AVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLTERARLAGAVNTLKKDDGEILGDNTDGEGLVQDLLAQQVLLKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQ---LAELVAAYG-EVKAQAFEQLKQ------SYDVIINSTSA---------IDPVIFSSRSVCYDMMYG-----------------IDGLGMLVGQAAESFMLWRGLRPGTKQILREL-----

General information: TITO was launched using: RESULT: Template: 3SEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1276 -62506 -48.99 -268.27 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -48.99 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_3SEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SEF-query.scw PDB file : Tito_Scwrl_3SEF.pdb: