Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNKDFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETAKKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNNAVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMPGISISTSWDRKVL-ETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQYEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFNSELENGGAKYSEGVYAVALNPKTGAVLSMSGIKH-DLKTG-ELTPDSL--GTVTNVFVPGSVVKAATISSGWENGVLSGNQTLTDQS-IVFQGSAPINSWYTQAYGSFPITAVQALEYSSNTYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFVPK--EYSFANYITNAFGQ---------FDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGDLIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTAESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNL----TNGVGPSIARDIINLYQKYHPMN 4R23 Chain:A ((50-475)) -----------------------------------------------------KSAYVQRGAIITSDGVTLAESVKQD-------------------------------------------------------------------------------------------------------------------------------------------DGTYVRNYPHDGMASHTVGYISTQ----------------------YGTAGIESSMNETLTGH------R-S---ALY-S-MAGI-TTGSSVVLTINSQMQAVAEAALQG--------------YSGSIVVMDPSTGAVLAKASSPSYTHAELGTI--SQLVDRTTQALYSPGSSFKTVTLAAGIDTHKTTLDTTYSAPGTMEIGG-GTIHN-YANE-DMGTIPLREAFARSSNTALAQLGVA---------------LGAD---NLVSYARAFGYGTALGQDFSTT-PSLMPNPAEMTTWELAWASCGLPVGEHASPAGPQTTVMQNAVIAAAIANGGVVMNPYIVDRVLSPE-----GAVVSTTSPKSLGQ-AVSADTAAQVREAMLGVVES--GTGM-G-AR-VPGVKIAGKTGTADVEN----GNFNSFFIGFAPYDHPTLVVSVVIEGNGENVLGYGAQVGGRVLAQCLNI--------

General information: TITO was launched using: RESULT: Template: 4R23.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2452 -4578 -1.87 -11.68 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -1.87 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_4R23.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4R23-query.scw PDB file : Tito_Scwrl_4R23.pdb: