Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQETKLSAKGPTKK--HVEILEELFPGYENTWRSSQEPA-RKGYAGTMFLYKKELTPTISFPEIGAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEER-QVWDAKYAEYLAELDKEKPVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPERYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL 3G4T Chain:A ((4-251)) LKIISWNVNGL---------RAVHRKGFLKWFMEEKPDILCLQEIKAAPEQLPRKLRHVE-------GY----RSFFTPAERKGYSGVA-MYTK--VPPSSLREGFGVERFDTEGRIQIADFDDFLLYNIYFPNGKMSEERLKYKLEFYDAFLEDVNRERDSGRNVIICGDFNTAHREIDLARPKENSNVSGFLPVERAWIDKFIENGYVDTFRMFNSD-PGQYTWWSYRTRARE-RNVGWRLDYFFVNEEFKGKVKRSWILSDVMGSDHCPI------

General information: TITO was launched using: RESULT: Template: 3G4T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1248 29701 23.80 121.72 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : 23.80 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.438

(partial model without unconserved sides chains): PDB file : Tito_3G4T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G4T-query.scw PDB file : Tito_Scwrl_3G4T.pdb: