Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVVVFSQIDEEILSRLQQDYHVVVLNPKLGDINEQIRQEVVDADGMIGAGRLLNESNLSPAQKLKIISSVTVGYDNYDVGYLNQKKIWLANTPHVLTETTADLAFTLLLSAARKVPFLDHWTKQGEWKRTVGPQQFGLDVFGKTLGIIGLGNIGAAIARRGFYGFNMNIVYHNRREKPELAEPLKAQYLGLEELLQQSDFVVTAVGLNAESKALMGKAQFELMQKHAIFINIARGSVVDEQALIEALQNEVIFAAGLDVYEKEPLQES-ALFNLPNVVTLPHVGSATAETRKKMANLAYKNLVEALEDKTPRYLVNPNFV 2CUK Chain:C ((63-306)) --------------------------------------------------------------AKGLKVIACYSVGVDHVDLEAARERGIRVTHTPGVLTEATADLTLALLLAVARRVVEGAAYARDGLWKAWHPELLLGLDLQGLTLGLVGMGRIGQAVAKRAL-AFGMRVVYHARTPK-----PLPYPFLSLEELLKEADVVSLHTPLTPETHRLLNRERLFAMKRGAILLNTARGALVDTEALVEALRGH-LFGAGLDVTDPEPLPPGHPLYALPNAVITPHIGSAGRTTRERMAEVAVENLLAVLEGREP---------

General information: TITO was launched using: RESULT: Template: 2CUK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1153 -167695 -145.44 -690.10 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -145.44 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.508

(partial model without unconserved sides chains): PDB file : Tito_2CUK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2CUK-query.scw PDB file : Tito_Scwrl_2CUK.pdb: