Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCMGRLAVNLSMIFTE--VPLIERFALAHAEGFEHVEIQFPYEL-----AISDIQDQLERYNLNLCLINVPAGDLMQGGNGLAG-IPGQEAAFREALQLAIRYATALKVPRVNILAGKQPQ------DSDLLPCLKTLASNLKLACDLLTEHDIEPVFEMINGTDMPRFLVQNIAQAQEMLEAVNHPALKMQYDCYHMAMMGEDVLEGLQENIHQIGHIQFADCPGRHEPDTAQIPYEQIFSWIKQSAYEGYIAAEYKPKN-------------------------GSNQSFTWKKKYFSDDVNI 4XSL Chain:D ((1-291)) --MNKVGMFYTYWSTEWMVDFPATAKRIAGLGFDLMEISLGEFHNLSDAKKRELKAVADDLGLTVMCSIGLKSE-----YDFASPDKSVRDAGTEYVKRLLDDCHLLGAPVFAGLTFCAWPQSPPLDMKDKRPYVDRAIESVRRVIKVAEDYGIIYALEVVNRF--EQWLCNDAKEAIAFADAVDSPACKVQLDTFHMNIEETSFRDAILACKGKMGHFHLGEAN-RLPPGEGRLPWDEIFGALKEIGYDGTIVMEPFMRKGGSVSRAVGVWRDMSNGATDEEMDERARRSLQFVRDKLAG----

General information: TITO was launched using: RESULT: Template: 4XSL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1378 18261 13.25 72.46 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain D : 0.67 3D Compatibility (PKB) : 13.25 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.474

(partial model without unconserved sides chains): PDB file : Tito_4XSL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XSL-query.scw PDB file : Tito_Scwrl_4XSL.pdb: