Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRQQIDVLVKQMNIDTA-KGEVDARVQQIVVRLLGDLFQAIEDLDIQPSEVWKGLEYFTDAGQANELGLLAAGLGLEHYLDLRADEADAKAGMTGGTPRTIEGPLYVAGAPESVGFAHMDDGTETGKIDTLIIEGTVTDTDGNIIENAKVEVWHANSLGNYSFFDKSQ--SDFNLRRTIFTDADGKYVALTTMPVGYGCPPEGTTQALLNKLGRHGNRPSHVHYFVSAPGYRKLTTQFNIEGDEYLWDDFAFATRDGLVATAVDVTDPAEIQRRGLDHAFKHITFNIELVKEATAAPSTEVERRRASA 1DMH Chain:B ((6-309)) FNTQDVQDFLRV-AS-GLEQEGGNPRVKQIIHRVLSDLYKAIEDLNITSDEYWAGVAYLNQLGANQEAGLLSPGLGFDHYLDMRMDAEDAALGIENATPRTIEGPLYVAGAPESVGYARMDDGSDP-NGHTLILHGTIFDADGKPLPNAKVEIWHANTKGFYSHFDPTGEQQAFNMRRSIITDENGQYRVRTILPAGYGCPPEGPTQQLLNQLGRHGNRPAHIHYFVSADGHRKLTTQINVAGDPYTYDDFAYATREGLVVDAVEHTDPEAIKANDVEGPFAEMVFDLKLTRLVDGVDNQVVDRPRL--

General information: TITO was launched using: RESULT: Template: 1DMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1368 24258 17.73 80.59 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain B : 0.86 3D Compatibility (PKB) : 17.73 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.599

(partial model without unconserved sides chains): PDB file : Tito_1DMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1DMH-query.scw PDB file : Tito_Scwrl_1DMH.pdb: