Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLDALNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQCGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTDQRIQHVANQASGFIYYVSLKGVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAAS-AKAMGSVADAVIVGSAFVKSFATLAADEAVEQTVNKVKELRAALDELV 4Y6G Chain:A ((1-259)) MERYENLFAQLNDRREGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLADGPTIQNANLRAFAAGVTPAQCFEMLALIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVEESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSG-----------LHHLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVK---------IIEKNLASPKQMLAELRSFV

General information: TITO was launched using: RESULT: Template: 4Y6G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1351 -163742 -121.20 -662.92 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -121.20 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.594

(partial model without unconserved sides chains): PDB file : Tito_4Y6G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y6G-query.scw PDB file : Tito_Scwrl_4Y6G.pdb: