Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEHRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRAQKLHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCPFCDVAHGRP----NALDPDEPRHMAETIANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNTLLEILVPDFRGRMDIALRIMTECPPDVFNHNIETV-PRLYKAMRPGSDYQHSLNLLKMFKEYCPDIPTKCGLMVGI-GETEEEVIALLDDLRAHDVDYVTIGQYL-QPSKQHAPIDRFVTPEEFERYAEHGRKLGFRNIWSAPMVRSSYFADRQYYGEPVPEVRRKVDPAKKIAVQAIEA 5FEW Chain:A ((64-247)) ---------------------------------------------------------------------------------RAIIEFSNVCRKNCLYCGLRRDNKNLKRYRMTPEEIVERARLAVQFGAKTIVLQSGEDPYYM---PDVISDIVKEIKKMG--VAVTLSLGEW--PR-EYYEKWKEAGADRYLLRHETANPVLHRKLRPDTSFENRLNCLLTLKEL--GYETGAGSMVGLPGQTIDDLVDDLLFLKEHDFDMVGIGPFIPHPDTP---------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FEW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 868 11612 13.38 65.60 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 13.38 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_5FEW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FEW-query.scw PDB file : Tito_Scwrl_5FEW.pdb: