Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTNKKNLGIGGIIVLLIAAYLGLDLSGHKNNPTPSSIPVNQRTETTFSNDGVDTIKAAYERRQSNVQVQGSGRVKAILREDNDGSRHQKFILVLKNGLSILVAHNIDLAPKIEDLRKGDIVEFNGEY-EYNEKGGVLHWTHHDPQNRHENGWLKHNGRIYQ 3B4Q Chain:A ((4-93)) ------------------------------------------------LNSAPTPRDVVANAPAPVQAAVAGAQEYAAQAGLN--TEELAVDALYNAIKVRLAGGIP--PQIEAFYQANRTNFNGFYMA-NRGAIDFIFS---------------------

General information: TITO was launched using: RESULT: Template: 3B4Q.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 333 -18376 -55.18 -213.67 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -55.18 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.169

(partial model without unconserved sides chains): PDB file : Tito_3B4Q.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3B4Q-query.scw PDB file : Tito_Scwrl_3B4Q.pdb: