Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISMKVVFHIDEL--EKWSETGKNVKNLIKASPETTIVVSVNGIAITGYLDSANA----------EFLDLQGV-VFHACANAMRANHISESSLPEQVI------------VVPAGVLDLVELQSQGYAYIKP 2HY5 Chain:B ((205-336)) VKKFMYLNRKAPYGTIYAWEALEVVLIGAAFDQDVCVLFLDDGVYQLTRGQDTKGIGMKNFSPTYRTLGDYEVRRIYVDRDSLEARGLTQDDLVEIAFEDMETEEEFDNIVEVIDSARVSELMNESDAVFSF

General information: TITO was launched using: RESULT: Template: 2HY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 413 -286 -0.69 -2.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -0.69 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.422

(partial model without unconserved sides chains): PDB file : Tito_2HY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HY5-query.scw PDB file : Tito_Scwrl_2HY5.pdb: