Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAESAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQQKVAEVQQLYKDFHVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS--DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQPKWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIVLEVTDSGVGMNEETRDRMFEKFYQGDTSR--QISGNGLVTFTG 4KP4 Chain:B ((9-194)) -----------------------------------------------------------------------------------------------------------------------------ADDRTLLMAGVSHDLRTPLTRIRAYAETIYNSLGELDLSTLKELAESINKDIEECNAIIEQFIDYLRTGQ---EMPMEMADLNAVLGEVIAAESGY--EREIETALYPGSIEVKMHPLSIKRAVANMVVNAARYGN--GWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVRGDSARTISGTGLGLAI---

General information: TITO was launched using: RESULT: Template: 4KP4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 577 15576 26.99 85.58 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : 26.99 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_4KP4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KP4-query.scw PDB file : Tito_Scwrl_4KP4.pdb: