Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------METTDTLLQLLQEAHKTNQAQQQTIQNL-----TTEIQLLNEKVNYLTNKLFGRSKETLFEETN-GQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGRT---KVREEIRFHQAMLDCLTHYQHTYC--------CKSCEKEGLSSFKKAIVP-----KPLISNSLGSNSLVAETI--RMKFGQKVPAYRQENYWKQTHGLDISR--DNITNWHIKAVQNALDPLGERLRVNNNQEEIL-----HGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESR--AGDVPKTFLKEFTGYLHC--DGYSGYNAVESVRLVYCFAHVRRKFFEAIPKEKKNTDIPAAQAVKQLD--------KWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN 3L2P Chain:A ((1-579)) HMRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLT--IQEVDEFLLRLSKLT-KEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVELSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAF---------QDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEG------LVLKDVKGTYEPGKRHWLKVKKDYLNMADTADLVVLGAFYGQG--SKGGMMSIFLMGCYDPGSQKWCTVTKCAGGH-----------DDATLARLQNEL---DMVKISKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWK----SATNLPQLKELYQLSK------

General information: TITO was launched using: RESULT: Template: 3L2P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2224 74901 33.68 159.03 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 33.68 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_3L2P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L2P-query.scw PDB file : Tito_Scwrl_3L2P.pdb: