Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELEEKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAYRKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN 2PIH Chain:B ((3-122)) -------------LYSKKDIVQQARNLAKMISETEEVDFFKRAEAQINENDKVSTIVNQIKALQKQAVNLKHYEKHEALKQVEAKIDALQEELEEIPVIQEFRDSQMEVNDLLQLVAHTISNQVTNEIITSTG----

General information: TITO was launched using: RESULT: Template: 2PIH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 227 -16575 -73.02 -138.13 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -73.02 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.725

(partial model without unconserved sides chains): PDB file : Tito_2PIH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PIH-query.scw PDB file : Tito_Scwrl_2PIH.pdb: