Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MREYKGLKIAVAGTGYVGLSIATLLAQSH---TVTAVDIIPEKVELINNKKSPIQDDYIEMYLAK-KELDLMATVDGVSAYKDADFVVIAAPTNYDS----KKNFFDTSAVEAVIKLVME-VNPEAIMVIKSTIPVGYTASIREKTGSK-------NIIFSPEFLRESKALYDNLYPSRIIVGTDMEDERLVEAAQTFAKLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRVSYFNELDTYAEMKGLNTQQIINGVCRDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYE----DVPENLIEAIVESNRTRKNFIADRVLELAGAYEANSHWDASKEKKVVIGVYRLTMKSNSDNFRQSSIQGVMKRVKAKGTTVVIYEPS---------LKDGEIFFGSKVVNDLDQFKAMCDSII-----ANRYDTCLDDIKEK-------VYTRDIFQRD------------------ 2Q3E Chain:A ((2-467)) --MFEIKKICCIGAGYVGGPTCSVIAHMCPEIRVTVVDVNESRINAWNSPTLPIYEPGLKEVVESCRGKNLFFSTNIDDAIKEADLVFISVNTPTKTYGMGKGRAADLKYIEACARRIVQNSNGYKIVTEKSTVPVRAAESIRRIFDANTKPNLNLQVLSNPEFLAEGTAIKDLKNPDRVLIGGD-ETPEGQRAVQALCAVYEHWVPREKILTT--NTWSSELSKLAANAFLAQRISSINSISALCEATGADVEEVATAIGMDQRIGNKFLKASVGFGGSCFQKDVLNLVYLCEALNLPEVARYWQQVIDMNDYQRRRFASRIIDSLF----------NTVTDKKIAILGFAFKKDTGDTRESSSIYISKYLMDEGAHLHIYDPKVPREQIVVDLSHDQVSRLVTISKDPYEACDGAHAVVICTEWDMFKELDYERIHKKMLKPAFIFDGRRVLDGLHNELQTIGFQIETIGKKV

General information: TITO was launched using: RESULT: Template: 2Q3E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2057 16809 8.17 41.92 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 8.17 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2Q3E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q3E-query.scw PDB file : Tito_Scwrl_2Q3E.pdb: