Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------MNDFTTEIMEILI------------------NKGDLDDLFRRHLELAINALLQAELTVFLDYEKY-DRAGFNSGNSRNGNYSRSFKTEYGELNL-VIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKG-----ITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKER--TLEAKYSVIFMD------ATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLL--VVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQAS-SKEEANNQIRFMIDKWKKQYPRVVKLLMN---PAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF 5C3M Chain:B ((5-438)) LKMATIGGGSSYTPELVEGLIKRYHELPVGELWLVDIPEGKEKLEIVGALAKRMVEKAGVPIEIHLTLDRRRALEGADFVTTQFRVGGLEARAKDERIPLKYGVIGQETNG---PGGLFKGLRTIPVILDIIRDMEELCPDAWLINFTNPAGMVTEAVLRYTKQEKVVGLCNVPIGMRMGVAKLLGVDADRVHIDFAGLNHMVFGLHVYLDGVEVTEKVIDLVAHPLGWEPDFLKGLKVLPCPYHRYYYQ-TDKMLAEELEAAKTKGTRAEVVQQLEKELFELYKDPRGGA---------------YYSDAACSLISSIYNDKRDIQPVNTRNNGAIASIPPESAVEVNCVITKDGPKPIAVGDLPVAVRGLVQQ---IKSFERVAAEAAVTGDYQTALVAMTINPLVPSDTIAKQMLDEMLEAHKE---HLPQFF

General information: TITO was launched using: RESULT: Template: 5C3M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1976 84659 42.84 232.58 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : 42.84 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.115

(partial model without unconserved sides chains): PDB file : Tito_5C3M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5C3M-query.scw PDB file : Tito_Scwrl_5C3M.pdb: