Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKISTYENKKVLVLGLAKSGVS-AAKLLHELGALVTVNDGKPFDENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITEVELAYEVA---ECPIIGITGTNGKTTTTTMTGLLLNAGADQGI--ARLAGNIGYPASGVAQEAKSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHG-TRKEYVKAKWNLQKNMTEKDYLILNWNQSELQELAQRTKARVLPFSTKE-VL--E-DGVYAD--DYSIYYKKEKIMEISELGVPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGE--IQGRKFYNDSKATNILATKMALSGFET----SKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTK--EKLMDAGKKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVNRLK 1J6U Chain:A ((0-434)) --------HMKIHFVGIGGIGMSAVALHEFSNGNDVYGSNIEETE-RTAYLR--KLGIPIFVP-HSADNW-YDPDLVIKTPAVRDDNPEIVRARMERVPIENRLHYFRDTLKREKKEEFAVTGTDGKTTTTAMVAHVLK-HL-RKSPTVFLGGIMDS-L-EHGNYEKGNGPVVYELDESEE-FFSEFSPNYLIITNARGDHLENYGNSLTRYRSAFEKISRNT---DLVVTFAEDELTSHLG------DVTFGVKKGTYTLEMRSASRAEQKAMVEKNG-KRYLELKLKVPGFHNVLNALAVIALFDSLGYDLAPVLEALEEFRGVHRRFSIAFHDPETNIYVIDDY-AHTPDEIRNLLQTAKEVFENEKIVVIFQPHR-----GNFAKALQLADEVVVTEVYDSGKMIWDSLKSLGK-EAYFVEKLPELEKVI-S-VSENTVFLFVGAGDIIYS-------------------

General information: TITO was launched using: RESULT: Template: 1J6U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2270 51625 22.74 130.04 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 22.74 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_1J6U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1J6U-query.scw PDB file : Tito_Scwrl_1J6U.pdb: