Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------GPSGVKA--ESSVRNPKYGDHCELTVWIHYKGKDIPLMRYYVEIGGDLTFDEKDGAWKHCVIKTTKQACQATWTGGSKSEP-CPPKNINVDARKVIQQHEFESESDTWWKRDI---------------------------------------------------------------------------- 1CHM Chain:A ((1-401)) QMPKTLRIRNGDKVRSTFSAQEYANRQARLRAHLAAENIDAAIFTSYHNINYYSDFLYCSFGRPYALVVTEDDVISISANIDGGQPWRRTVGTDNIVYTDWQRDNYFAAIQQALPKARRIGIEHDHLNLQNRDKLAARYPDAELVDVAAACMRMRMIKSAEEHVMIRHGARIADIGGAAVVEALGDQVPEYEVALHATQAMVRAIADTFEDVELMDTWTWFQSGINTDGAHNPVTTRKVNKGDILSLNCFPMIAGYYTALERTLFL--DHCSDDHLR------LWQVNVEVHEA---GLKLIKPGARCSDIARELNEIFLKHDVLQYRTFGYGHSFGTLSHYYGREAGLELREDIDTVLEPGMVVSMEPMIMLPEGLPGAGGYREHDILIVNENGAENITKFPYGPEKNIIR

General information: TITO was launched using: RESULT: Template: 1CHM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 388 19447 50.12 196.43 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : 50.12 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.407

(partial model without unconserved sides chains): PDB file : Tito_1CHM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CHM-query.scw PDB file : Tito_Scwrl_1CHM.pdb: