Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVRKALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDKIIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTR-QIAEQSIYSYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTESRHKLEKFRFVDFAKRKK 3EDP Chain:B ((9-235)) -PLFEVIASKIKDSINRDEYKTG--MPNETALQEIYSSSRTTIRRAVDLLVEEGLVVRKNGVGLYVQPKLTAQ-N---ILEMTGVM------LKKDIKDFYIRKAGKFYAEIFGMKENELVYSIKFVQKS-EHGATLDRLILPLGLYPDLQAKDFQIINIIELVN-SGKYKLFELEQELQLILAGNEQIKNMHLNENDPVFKLSSVFYAENDMPIAIQYHYEDAESTKYVVDF----

General information: TITO was launched using: RESULT: Template: 3EDP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1014 -49642 -48.96 -228.76 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : -48.96 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.311

(partial model without unconserved sides chains): PDB file : Tito_3EDP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EDP-query.scw PDB file : Tito_Scwrl_3EDP.pdb: