Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDTSKFNQFVRDIYQTNNFIPLHEPRFLGNEKKYVLDTIDSTFVSSVGAYVNDFESKIQHFTGCAKAIATVNGTAALHIALLLAGVKRDDIVITQALTFVATCNALSYIGAEPLFIDVSLKTMGLCPKALDTYLQENAFLDDQGVCKHTATNKRISAIVPMHTFGHPVEIDELQEVVTRWNIALVEDAAESLGSYYKGKHTGIFGLVSALSFNGNKVITTGGGGMVLCQDEELGVRAKHITTTAKIPHP-YEFYHDENGFNYRLPNLNAALGCAQMESLEGFLSKKRDLAHQYQNFFKDT-----DISFFVEPENCQSNYWLNAIICKNK---TQRDLILDETNSNKVMTRPIWTLMTRLPMYENA--LQGDLTNSLWLEERVVNIPSSVPLE 4LC3 Chain:B ((14-370)) ----------------APFLPFTRPEIDEETIQGVVEVLRSGWI-TTGPQCQKFEAALSEYCGGRPVRVFNSGTCTLEIGLRIAGVGPGDEVITTPASWVSTSNVIIETGATPVFADIDPVTRNIDLDKLEQAITP-----------------RTKAIIPVFLSGLPVDMDRLYAIARAHKLRVIEDAAQAFGSTWHGKRIGAIGDLVSFSFHANKNLTTIEGGALVLNNEDEAVLAQKYRLQGITRTGFDGMDCDVLGGKYNLTDVAARVGLGQLPHLERFTAQRRALARAYFAAFDGGAAAKLGVGLPVAE-FENGNWHMFLVTLPLERLTITRAEFMAQMKERGIGTGIHYPAIHLFTLYRARGFKEGMFPHAERYGASTVTLPLFTQM-

General information: TITO was launched using: RESULT: Template: 4LC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1877 -155590 -82.89 -449.68 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -82.89 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_4LC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LC3-query.scw PDB file : Tito_Scwrl_4LC3.pdb: