Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIVKRSFNMTFAIKKIHHVAYRCKDAKETVEWYKKMLNMDFILAFAEDHVPSTKAFDPYMHLFLDAG-QGNVLAFFELPTQPEMGRDENTPQWVQHIAFEVEDLNALLAAKKHLEDNGVKVLGITNHGIFHSIYFFDPNGHRLELTYNDAHAEEKIAKIT-EEMKTEMLEEWSKTKRAPHHTHFLHEEELGA 3OAJ Chain:B ((147-304)) ---------PDVAIKGFGGATLLSEQPDKTADLLENIMGLERVGKEGDF------------VRYRSAGDIGNVIDLKLTPIGR----GQMGAGTVHHIAWRANDDEDQLDWQRYIASHGYGVTPVRDRNYFNAIYFREHGEILFEIATDPP--GFAHDETQETMGEKLMLPVQYEPHRTQIEQGL--------

General information: TITO was launched using: RESULT: Template: 3OAJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 559 2149 3.84 13.77 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain B : 0.67 3D Compatibility (PKB) : 3.84 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.448

(partial model without unconserved sides chains): PDB file : Tito_3OAJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OAJ-query.scw PDB file : Tito_Scwrl_3OAJ.pdb: