Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHSDTLSLSLELLQQPSVTPIDHTCQTIMADRLAKVGFHIEPMRF-------------------GDVDNLWARR-GT-EGPVFCFAGHTDVVPTGRLDAWNSDPFAPEIRDGKLYGRGSADMKTALAAMVVASERFVAKHPNHKGSIAFLITSDEEGPAVNGTVK-VIETLEKRNEKITWCLVGEPSSTHKLGDIVKNGRRGSLNAVLKVQGKQGHVAYPHLARNPIHEASPALAELCQTVWDN-----GNEYF----PATSFQISNIHAGTGATNVIPGTLEVTFNFRYSTEVTAEQLKQRVHEILDKHGL--------QYEIVWNL-SGLPFLTPV-GELVNAAQTAILNVTGTETELSTSGGTSDGRFIA-PTGAQVLELGVLNATIHQINEHVDVHDLDPLTDIYEQILENLLA 3PFO Chain:B ((24-429)) -NFNDQVAFLQRMVQFRSVRGEEAPQQEWLAQQFADRGYKVDTFSLADVDIASHPKAAPMDTIDPAGSMQVVATADSDGKGRSLILQGHIDVVPEGPVDLWSDPPYEAKVRDGWMIGRGAQDMKGGVSAMIFALDAIRTAGYAPDARVHVQTVTEEEST-GNGALSTLMR-----GYRADACLIPEPTGH-TL----TRAQVGAVWFRLRVRGTPVHVAYSETGTSAILSAMHLIRAFEEYTKELNAQAVRDPWFGQVKNPIKFNVGIIKGGD-WASSTAAWCELDCRLGLLTGDTPQEAMRGIEKCLADAQATDSFLSENPAELVWSGFQADPAVCEPGGVAEDVLTAAHKAAFNAPLDARLSTAVNDTRYYSVDYGIPALCYGPYGQGPHAFDERIDLESLRKTTLSIALFVAEWCG

General information: TITO was launched using: RESULT: Template: 3PFO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1919 30031 15.65 82.50 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 15.65 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_3PFO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PFO-query.scw PDB file : Tito_Scwrl_3PFO.pdb: