Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQARDQA--PYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGID----RIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK 4LC3 Chain:B ((14-387)) --------------APFLPFTRPEIDEETIQGVVEVLRSGWI-TTGPQCQKFEAALSEYCGGRPVR-VFNSGTCTLEIGLRIAGVGPGDEVITTPASWVSTSNVIIETGATPVFADIDPVTRNIDLDKLEQAIT------PRTKAIIPVFLSGLPVDMDRLYAIARAHKLRVIEDAAQAFGSTWHGKRIGAIGDLVSFSFHANKNLTTIEGGALVLNNEDEAVLAQKYRLQGITRTGFDGMDCDVLGGKYNLTDVAARVGLGQLPHLERFTAQRRALARAYFAAFDGGAAAKLGVGLPVAE-FENGNWHMFLVTLPLERLTITRAEFMAQMKERGIGTGIHYPAIHLFTLYRARGF--KEGMFPHAERYGASTVTLPLFTQMTEGDVRRVVDAVNQICE

General information: TITO was launched using: RESULT: Template: 4LC3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2124 -106038 -49.92 -288.15 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -49.92 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_4LC3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LC3-query.scw PDB file : Tito_Scwrl_4LC3.pdb: