Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESFLANVDSAHLKARLNKIMADVNAVTSDAAAAAVSAKSSEEAGQCVLEFVKFFVSVAEQHGTTPHAVLGQHVVSNVLIFVLERIVKPNLMTYCRVAEKHAAIAVAASGDTTSPARCRAAKLGSGAASAATGRRDALEARRRRLRRDIIRPFAQLFQSLHTSLAEEGEPLMTWATEGGGRTLGATRRAAALRSGGRHEELLGSASPSYAPPESSSAFGGSRRGEQRAGEWRGRDDVWGTGDSAHEDGLLISGASDALSQAPLLWKSASPLNILCNHILEVLESQWLVAAVGADYAALLIEVLRWSGYARLITPTYLVRLSECLVTLIQRVSTLEEADTDVLTVAPNLVAQAVADGRLWVAAPCADDACLYAQVLMRLCALPQITTAYPPSSSTATDAGMHGGGGAAAALAFVRRVIEVVKARHRAPTPDLRLEAYCIAALRRLCQRFRYDHPIAARAPVEVVKLLCDFFFLLHHTPRHDVWRLETMQFLTVVLSVALSDVVGEELWQDLHAHSRRAAADADAEAAMVSPDAREMVHKAKSSERVGLVGRRGTTGAATAANLLLQPPTRHLVVLIHQSVFPTMQLIFTQHRHEYSFTSRRELFSFPCSPLQEVFDFGAVALYVTSITHGAYCRWKAAQANRCGGSGTTRCGTVADQEKEGAEDDEDEEQIRRHGAGTEARRACKRLRDIEPGYGDVVDGVAMDKAADASGAARTPPPASPHPAQLLADTLQEEFFMPAGPLESSTTAGRAATAGHDVQQRPLQSAATLTRRRAGGAPLVLRIGTSSIRTSSAAVQDVVSDTALFLLHLFAQPCATLRVPETVADRLVEDVLLPLCGHFGLRLGQLLLAAVKAVIPRCSLATQQYAFAVVAQRTLPAVPPALARWAGCLDSVSSSRVPVTRNDEDVLAPPEPVYHVLVVLLEQHLHRRHELGLSTVTPDDPPRNGQSAGAAGSGQPDVSSGRLLSGFLLSWGDHHRALIQRGVARLHAFEQDMRRVCESAGATAVPAGVGGAHDAPLPLLAASLALLLTRFATLVQCTRLWEMTLCPSTAMPVDPGTTEAEPQRCRVVHDDHTWGLSDGPAEDVGLSFLCHVAANATLFLGSVPVLTRASDGGSHAGDVAGGGVRAARQASGIGTDALTATSASLETRNALTGADAVALFSAAQFTAWLDLAETYACVALRMCLARQQVAEQTEMATSLRLGQGPALGGVPSLSTSSGAMTPHLRGPLRREVGAYVEHVCTLLDAAVTSPSSQVPGVLRDAAEAHWMLRLPLRKGTDRTDVQDGSAAAEEGAAMGEDAKADTDAAALGTGVSPLQMTVPRRAALQAFQAMLASSAQASVAWLMRRAQLRHTLAALAPAAVSRREGNRRGGGGPPDTHTAVRPDLDASAMALALSVQCVQLLHLLLRWRRAALWSTPEESAHARDGVHAAGERGAVTPGVAKDAPFLRSAADGTHASAASERAEGAAADFDVGEEASAACEDLRLLRSSPDVYAAPLSLLERFSAAGGGRLYYAVLWLCHHIAAELDHAHAVGAFFPLWTALHVLFRDVGTVLLDPAFACADPRHTIARFTAALTARIFEDGVRGLSRWRPSNPTSREVTHIMAVSLHMSVDTLRTVLWAASRTRIGAYLAEPLADTSAICRTIVTMLRDQHLLDTAGADSCLTGDAARGSGESDAAAVGDILLPSHHRLLERLPFLPTILLALPPLLRLLALIGCLPPPPADSDTGAAGGFQCCRAVVQVLQVLFVRYRYQLGPGLCSGLLCGTVADIRWLDFRRHPATLRDAGERDSQGCSDAAAFLYAHEGRLLELWTAQLTDHSRVDMPVCTPAWQCALLQAAFAVLPRCEPAVAEVLGRFSFEFLAEKSPYEVRHHAALQVGTLFRTFSARRTQVLHTLLTKAREGMLSSTETLCSTSLLALSEAVRAAPELLVDVLYTLLECWATRGFRHHRLVVACLERMSASVLEGARLTAPAGGASLPRSLPYTHLCRMHARPLLFKWLCEYRHPHAALPVECFGYPTQTAFVVQHLDVLSPLALLLLTEATEEQWSLVATRGGGGRCDGASAEGSLYERLVSAYADTLVAKAAEESEHAAEALGAARRRRRTEASGSDATAKQDEATEAESQTDATAVSDAGALHRLAPLCMLLAYFPGIVTQLLVFASHAPVSPFAGDAGGLADLGKARNSREFADWPRRMGHADAAVEHRGSGGGHSSRASASDMTPTWASVAQRCLYWLEAQLNTAEATMLAQGVRLVLTGRRHVSGESPQPTFSAAAARAVLCHPATASSAAEVLLYYLQHRSAWPHKRRAFDVVLAAQADAVMEALIVLHRTTSEVGAPVYGTPEQLQRALSWVAEKITSVPLPMPGLRDPRTRHASSAEGRGSATAEVETTQGLVPRCTSSDLAHWEAYCLALLVPPAAAPNCELHSPVTDMPSARQSVDAAALTDDVMTRFLLQGNSTYLYALLHAVYRSSAALPSLELQFRALQQLSLLTHIATCWCSARVLTEPHALRLVLSHLLHWLRRSPAAAVRRLVCDALLHVWQVARAGRGNAPRHRGWGTTPATQRRHCRLMVARSLAPLTAVSPLISSTWAALCRIDPDLRRSAWQQRVLAYLADTESRRATRIGAETAAEGKERVLARGAISIDEPTDDEAERNEEGAASDGAPGASAGAGAAESEAVSGGCSLPLDGEQWTWSMYEAQRSACLAPLSNPRARGHLLGLQRLLRYVSGHDEEDSDEALEGVPGTVEEHGSNADMDELVPPESCAVSMQLIVEHTSAAPLPSAPLLQDITVAVDSAQALLVRC-LHVASVCLRAGHAPPVAAGPTAHRRSRVRTGGSLRPAELSALDVRAVLLDTIQHLYRLCHCNMAALPTEYADAADQFRPLLPGYVAGEDVHGVHTTRLQMLSADSADEQLLAAFRLLRALALCVVLSGEAATLPHCAAAVSSAPSGMGSGSEWLRHEAQRSGSSASTLLAASESALTDDGLPSNQTAEGIVAGMLAQAYAHQLRTLEQLVWCGDADASELGMCALRAWVLCIASGPDGADGAEPP-RPAEPPSCFDEAVAQLVASAWRQQLTGPSAAAAAGELVGASGGDQMPSPKARVCYCLQRRLSW-----------VTRSGSCGSRSCRQRATFGTRGAAADTHLRSPSVWAPLRQCPPNQRAFLCRFVLAAANTYGMLKKSLLWAALVPLLMAELRTAEGPTAVPTSSAGSPACDLDGVHAACDSAGSDVRIAEQLLLPVLLHALCLRESEGQRVVRQEWSRQLDRYVFQQAERCAHTARLFLHVLEQCHAVLLRSTRQRGLKGATAASAGRAKATSSFSAAAAAAAAGDGARAEVAPWPSILGSVPSLQDMGECYWLSDIPAARLARAAVRVGEPHLALLFSQLSGESLYGPRTGAAALCGESETTLSAVMLRDGAGAGEAVLAMSSTPYSILFPFARPGDYPSAASVTHTVGAGATRSGGRARDRHDAVRRQTRSFAEDIFDLYASVQGQLELDDVVGVSLMMRLQSVTSAQPHGDGAKWCSWVGSGSALSASLLGSARGRSSGRAIRRVVGYRESGVTPTTSVASGADDSLAAAPSATAAWLDEMRLLEESETHHASGRLWGSDVPRASAAADSGLGGATVEVARPSVALTGEVQERRPDPWAAHLQRAALLLQHGFPSTALDVLLSLQPAERRWEAAERHRSDSGAAERGVADDSVRACSAPGRAAGRGTTELGPEVFLTGAAGETIDAAAGNTDMLSSWTPAHEASLQALLAEAAWKCGRWSCGGGVAGDCPGSCSSECVPRAVLDRLSLRGNTATPESCGASLMPASAGRFYVHLLSAFITLTNGQPLLSMQHVRRAESALRQQLSPTTFVTTVMAA------EALHEVRDCAARFLAADAAARKGKAGKPSRGRGAAPKWKQGDRVVELPTWLRSLRGDSVARGSGLDAAELPTARVPSTVDYASRELLDSVRHALCQVYGDREGWYRFVLGATEQALLNRDAAAAHRWLRDWKEQHAEGTGGEGEQASPSPAIV--GTAAAEVRERARQLDLVLRTAQVAYGLGRWQEALALLQPAAAAARGEASLAHFNVAPPRTAPWHAPQEPRVI--QQLMVWHGELRLLP------PSQLVRDPFLSRAAASDSSGACSFLLARLCHTLADDIAARLTSHEH-----QQLCESVEESKRLKEDL--------EAQLEAATRPSTGRAAAPLHRPPGVAELDATTGATTTAVLPTLVNIAGVAPLREARGRSAVADAAAGAAAGAAPTVILSEDQLRVIRRRIRELAGDIKRLEDEWQSEKSNFGLYRRSAINAYSRFLQFNQVAQQGAARGVTERTHRGAAALSEVKCLPVDHEEDVVHAVFGFVELWVNAADMEQGGGEVLSKVLDKAVERIPTAVFVPLAGQLTAQLGGQHEGERLAFLVARLARDYPMHVVWPLLALYHGHTFAKSRDVNTLHNVDEAKITAARQLLAGLASAQSTANASSRAGHSASTAALLSTQIRHAQLLSSAYLELAFDRSAATAQVGKRHAICKDFMLIKDACNLAIPPPSSVDPFVTPAVAG--GAALAVPHVMRYRNYFTTPGGVNVPKVLQCELSDGTVVRQLLKAGDDLRQDALIEQVFATANRLFRR--RSATRPLQIRTFTIVPLAPTAGILQWVEHTIPLGEYLTGRYTGKEEMPGAHERYFPGEPNTRECRIQLQNAP-------HSRKCQVLLSLYAQFTPALHYFFLEEYLSAQVWVDRQQTFTRSVAASSIVGYTVGLGDRHINNILLHKGTAEVVHIDLGIAFDQNKLL-PVPELVPFRLTRNMIDGLGVRGTEGSLRPCAEAAMHLLRGKRELIRTILSSIMHDPLARWAIGSPTHA-------NHIMGADTSVTGAGAVPQESNTRQPAPVRTHGSSADAARTLARIDAKLRGYDGGDM--LSVPTHVRKLMEDAQRVESLAVMFPGWSQWV 5FVM Chain:A ((85-2469)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------NVKAFDLIFTKLNQLERVAASYELKSCLIS---LVSTEYFQRFSNDINNKVFELIHDSNEKLGGVLAVDTLIDELPNQTSRLANYLRVLIDIEVMRAAATTLGKLAVLTSEFVEFEVKTCIEWLTQEYRKHASLLIISSYPFVNSILDNIWRALRKLVIRTDAAVTLGKCLTSSLTKKWVQRLFNG----CIHGTLLVYRQLVSLELKDKYEEIYETTMKHRDNIIIRKEIYAIIPVLAADPKLFTQKYLDATMIHYLTLLLSDKGPILVSIGDIAYHIGPYLDAIVENLRDGLETKFKTRKEKDIFYCVSKLACAVRPLLAKYLNRGLLDASDHMQETLLVICEKISMEVTVNEKLLNIICLVLSGEKFRPPGSPTPMKSLSSETARAYRDQSLLRKTGTLDAMILRKALRMLSDIKPKYSLTEFIRRVIISYMEHDNMQVRKLAALTSCDLFVKSLYALNVVSEVLSKLL----------------TVAITDPVAEIRLEILKHLDRLSQPDNTKLLFMALNDEVFAIQMEAMKICGRLAV----------IPSLRKTLIQLLTELKHSKMTRKKEECASLLCTLISSSSDVTKPYLEPVIEIL-----------------LPSAVASTALKALGEISV------VGGEDMLPFLDELMPLIIDTFQDQSNSFKRGAALKALGQLAASSYP-ELLGVLINILSQNIRRETVRLIGILGALDPYKHREVDVALLMQGEEYYPTVVIGVLMKILKDSIHHSTVIQAIMHIFQTMFLKQIIPGFILVMHPPSLLELYFQQLSVLISIVKQHIRPHVTEIVDVISLTIISVIESLSRALEGEFNPHLPTILSLFLDVLENKKVVSVRILKSLVVFGPHLEE------YVHLVLPTIIKLSS--GNLKKAAIITIGRLSKNNALEMSSRIVQALVRVLNETEYVKATMNTLSLLLLQLNIDFTVFIPVINKTLTIYDRLVAKLLNNEPLPTKIIIDKDFDLPNKEMDDVKVASKKLPVNQMVLKNAWDCSQ----QRTKEDWQEWIRRLSIESSSHALRACSGLAGIYYPLARELFNASFAQYQEDLIQSLCAALSSTLLNLVEFMEHDDKPLPISISTLGEYAQRCHAYAKALHYKELEFIEEPSTSTIESLITINNQLHQTDAAIGILKHAQQHHDLQLKETWYEKLQR--------WDDALNAYNKREAEVTIGKMRSLHALGEWDQLSELAADKWASSEVKRAIAPLAAGAAWGLAQWDRVEQYIEVMKPQSPDKAFFDAILCLHRNNFEKAAEHIFSARDLLVTEMSALYN---RAYGVVVRTQMVAELEEIIQYKKLPQGSERRAMIRKTWNKRLLGCQNVDVWQRVLKVRSLVIKPKQDMKVWIKFANLCRKSGRLGLAQKTLNTLLEAPPPVVYAQLKYMWATGSQKEALNHLISFTSRMAHDLGLQHHIEDYTKLLARCFLKQGEWRVSLQPNWRLENPDAILGSYLLATHFDKTWYKAWHNWALANFEVTSSLTQRIKDDKVPPT--LNANDNFPPELVQRHVVPAIKGFFHSIALSQSS----LQDTLRLLTLWFKFGGIPEAAQAMHEGFGLIKIDNWLEVIPQLISRIHQPNQTVSRSLLSL---LADLGKAHPQALVYPLTVAIKSDS----VSRQRAALSIIDKMRMHSPKLVNQAELVSDELIRVAVLWHELWYEGLEDASRQFFGEHNTEKMFATLEPLHEMLKRGPETLREISFQNSFGRDLNDAYEWVLNYKRTKDINNLNQAWDIYYNVFRRISRKLPQLQTLDLQH-----VSPKLSAAKDLELAVPGTYHAGKPIVRITHFEPVFTVISSKQRPRRVIIKGSDGKDYQYLLKSHEDIRQDNLVMQLFGLVNTLLQNDPESFQRHLNIQQYPAIPLSPKTGLLGWVPN------------SDTFHVLIREHREASKIPLNIEHRIMLQMAPDYDNLTLLQKVEVFTYALDNTKGQDLYKVLWLKSRSSESWLDRRTTYTRSLAVMSMVGYILGLGDRHPSNLMLDRITGKVVHIDFGDCFEAAILREKYPEKVPFRLTRMLTYAMEVSGIEGSFRITCENVMMVLRDNKESLMAILEAFAYDPLINWGFDLPTHAVMEQTGIDLPLANPSELLRKGVISVEDAAKMELQQKAEVRNARATLVLKRIADKLTGNDFPRCQELSVPDQVDKLIQQATSVENLCQHYIGWCS--

General information: TITO was launched using: RESULT: Template: 5FVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 9647 -19195 -1.99 -9.34 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -1.99 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.176

(partial model without unconserved sides chains): PDB file : Tito_5FVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FVM-query.scw PDB file : Tito_Scwrl_5FVM.pdb: