Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLRVRLRVPKQWDRFQVNIDEELQQEELSRTLTEREIDDFNRRNLHSVSELSDHLRLLVFGSDECVTCSEDICAAVEYIVGPQRRWNSSCDEVCEFRRAISRAYLNEWKAFQSVHPSVPERLATRSLPQVFSVPPLQKAQMDLLMQMCPRNAETGDGSISMRLWYLDSAGSRCSTRRRFPFDTAADDAIRLTMSSAREFASMREKTDDTPLIFVFRVKGRNEYIYGSQPLINYRYIRHCVSKRAEIDLVVEPKEATLFAVPLFRPSYPSRTLASPSDADASKSSTSEICLWDLTSKLSIRIVGGFEKLAISASRMKDENVRDSSSLYFAVLVEAYVGTWRCCEPQITDWQLCENAKNSHSLAARVYWKDGGTVTFSIDLSNVPRELKLCFTLVASSTDRIVGARAATAEEVIAAVEVNTKKTDDQGHSVFFLGTAATQLFDYAAKMRMGMWRIYLWEGKTRANPIGLNSINPD-SNACTFEVEFPCFDKPVFFPSGRPPKRKEEDARRNFADREAALSSYLINNEVEQLRQLKRVLMYDPLVHLSSDDKTILWKYREMLLGQPKAMAKVMSAVNWLMPFDVYEAHCLLRRWSPLA-----AFDALELLDAHYADLAVREQAVEYIDKMSDYELRGCILQLVQVLKYEPYHYSALARFLLRRSLRSNHIIGHYVFWYLAAEVK-NLTICERHGLLLEELIKR--SPHRLDYVRQVYVCNELLKCALRVQRAPK------KDRVKCLRASLAKVR---FPHRFTLALNPSVECCAVDIDECKVMESKKFPLWLAFRN---HLDQDEHFFIIFKSGDDLRQDLLTLQLLEFMDSLWKASGLDLHLIPYGCISTGEGVGMIEVVLNSDTIANITRREGGAQAAFNVDPIINWLRQFNHDRGEVERCLWNFVLSVAGYTVATYVLGIGDRHNDNIMLRQDGTLFHIDFGHFLGNFKTKFGIKRETAPFIFTPMYLYAMGGS---SSPIFKYFVDVACRAYNALRRHSSALMMLFMLMLSTGIPELQTLEDIEWLRTVLLLNRTDEEASDHYKGLINDALGNFRTLLNDYIHIMVH 1E8X Chain:A ((1-951)) -------------------------AASEETLAFQRQLNALIGYDVTDVSNVHD-------------------------------------DELEFTRRRLVTPRMAEVAGRD------PKLYAMHPWVTSKPLPEYLLKKIT-------------NNCVFIVIHRSTTSQTIKVSAD----------------DTPGTILQSFFTKMANERDFVLRVCGRDEYLVGETPIKNFQWVRQCLKNGEEIHLVLDTPP---------------------------------------DPALDEVRKECDRKFRVKIRGIDIPVLPRTADLTVFVEANIQYG----------QQVLCQRRTSPKPFTEEVLW--NVWLEFSIKIKDLPKGALLNLQIYCQ-----------------------------------LLYYVNLLLIDHRFLLRHGEYVLHMWQLSGFNADKLTSATNPDKENSMSISILLDNHPIALEMPN-------------------------------QLRKQLEAIIATDPLNPLTAEDKELLWHFRYESLKDPKAYPKLFSSVKWGQQEIVAKTYQLLAKREVWDQSALDVGLTMQLLDCNFSDENVRAIAVQKLESLEDDDVLHYLLQLVQAVKFEPYHDSALARFLLKRGLR-NKRIGHFLFWFLRSEIAQSRHYQQRFAVILEAYLRGCGTAMLHDFTQQVQVIDMLQKVTIDIKSLSAEKYDVSSQVISQLKQKLENLQNLNLPQSFRVPYDPGLKAGALVIEKCKVMASKKKPLWLEFKCADPTALSNETIGIIFKHGDDLRQDMLILQILRIMESIWETESLDLCLLPYGCISTGDKIGMIEIVKDATTIAKIQQSTVGNTGAFKDEVLSHWLKEKCPIEEKFQAAVERFVYSCAGYCVATFVLGIGDRHNDNIMISETGNLFHIDFGHV---------------PFVLTPDFLFVMGTSGKKTSLHFQKFQDVCVKAYLALRHHTNLLIILFSMMLMTGMPQLTSKEDIEYIRDALTVGKSEEDAKKYFLDQIEVCRDKGWTVQFNWFLHLVL

General information: TITO was launched using: RESULT: Template: 1E8X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 4292 -189080 -44.05 -231.43 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -44.05 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_1E8X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1E8X-query.scw PDB file : Tito_Scwrl_1E8X.pdb: