Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGVETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNLYPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSANGETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENPQQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHMNPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSYTDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKES-PADWQVVTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQAK---DRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFTGVRHFRH 4EHI Chain:A ((23-534)) NAMRALLSVSDKEGIVEFGKELENLGFEILSTGGTFKLLKENGIKVIEVSDFT--------------------------------------ILGIDLVCVNLYPFKKTTIM-SDDFDEIIENIDIGGPAMIRSAAKNYKDVMVLCDPLDYEKVIETLKK-GQNDENFRLNLMIKAYEHTANYDAYIANYMNERFNGGFGASKFIVGQKVFDTKYGENPHQKGALYEF-DAF--FSANFKALKG-EASFNNLTDINAALNLASSFDKAPAIAIVKHGNPCGFAIKENLVQSYIHALKCDSVSAYGGVVAINGTLDEALANKINEIYVEVIIAANVDEKALAVFEGK-KRIKIFTQESPFLIRSFDKYDFKHIDGGFVYQNSDEVGEDELKNAKLMSQREASKEELKDLEIAMKIAAFTKSNNVVYVKNGAMVAIGMGMTSRIDAAKAAIAKAKEMGLDLQGCVLASEAFFPFRDSIDEASKVGVKAIVEPGGSIRDDEVVKAADEYGMALYFTGVRHFLH

General information: TITO was launched using: RESULT: Template: 4EHI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2407 -174190 -72.37 -370.62 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -72.37 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.494

(partial model without unconserved sides chains): PDB file : Tito_4EHI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EHI-query.scw PDB file : Tito_Scwrl_4EHI.pdb: