Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTET-------ESILGLGG--KVLVQLIQA-QE-SGE--VREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGADHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEPFILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVP-SASWIAAGDYHHHLAVNEWRGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWM----TSSKLEITDSDGIVTRIRLAR 3OAJ Chain:B ((3-270)) ----AKKTMGIHHITAIVGHPQENTDFYAGVLGLRLVKQTVNFDDPGTYHLYFGNEGGKPGTIITFFPWAGARQGVIGDGQVGVTSYVVPK---GAMAFWEKRLEKFNVPYTKIE-RFGEQYVEFDDPHGLHLEIVEREEGEAN-------------TWTFGEV---------TPDVAIKGFGGATLLSEQPDKTADLLENIMGLERVGKEGDFVRYRSAGDIGNVIDLKLTPI-G-R-GQMGAGTVHHIAWRANDDEDQLDWQRYIASHGYGVTPVRDRNYFNAIYFREHGEILFEIATD-

General information: TITO was launched using: RESULT: Template: 3OAJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1362 29560 21.70 118.24 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : 21.70 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_3OAJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3OAJ-query.scw PDB file : Tito_Scwrl_3OAJ.pdb: