Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDIKIKREEIMKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYHMTSNGFIDSIINS--KWVGLDNFRFLFSSRDAFIIT--------RNTVLYNL---GFIFLGLVVSVGIAIILSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFYADLGIWPYLLLFLGIWKGFGYSS--VMYYATI--------------MGIDPTY--YEAATVDGASKWQRIRNVTIPQLTPLVTVL-----TILAVGN--IFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGMAAAAGLYQSVVGLILVILSNLLARRVDPNSALF 4Q3O Chain:A ((31-347)) -EVPIPQS-ISAEFKAALAQY--------PTPSVEEARSFVPT--TAAQWRDYVQATNKMQKTKIKNMRKHYGVTVELLDIKGVTVRKITPKSLSPEFKDHVYIDIHGGAYVLFAGLPSIEEGILIAHRLG---IVVYSVDYRMPPAYPF-------PAALDDVKHVYRVLSQQYDANHIFMGGTSAGGGLLLAFVQGLIENGVATPRAIYAGTPWADLTKTGDSLYTNEGIDRILITYDGTLGASARLYAGNTPLTHPKLSPIYGDFTDFPPTFLVTGTRDMFLSDTVRVNRKMRDAG----VTTVLDVY--EGLSH-ADYLVSHQTPESQSVYRQLKRFLVGFT-----------

General information: TITO was launched using: RESULT: Template: 4Q3O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1459 -108770 -74.55 -389.85 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -74.55 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_4Q3O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q3O-query.scw PDB file : Tito_Scwrl_4Q3O.pdb: