Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGGVVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFAWAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETRDDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNL-EFSFSGLKSAFINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANKGLRERLATEIT--DVNVIIPPLRLCGDNAGMIAYASVSEWNKENFANLDLNAKPSLAFDTME 2IVO Chain:A ((1-307)) ---MLALGIEGTAHTLGIGIVSE-DKVLAN-VFDTL-TTEKG-GIHPKEAAEHHARLMKPLLRKALSEAGVSLDDIDVIAFSQGPGLGPALRVVATAARALAVKYRKPIVGVNHCIAHVEITKMFGV--KDPVGLYVSGGNTQVLALEG-GRYRVFGETLDIGIGNAIDVFARELGLGFPGGPKVEKLAEKGEKYIELP--YA--VKGMDLSFSGLLTEAIRKYRS-----GKYRVEDLAYSFQETAFAALVEVTERAVAHTEKDEVVLVGGVAANNRLREMLRIMTEDRGIKFFVPPYDLCRDNGAMIAYTGLRMYKAGISFRLE-------------

General information: TITO was launched using: RESULT: Template: 2IVO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1815 -153962 -84.83 -506.45 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -84.83 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2IVO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IVO-query.scw PDB file : Tito_Scwrl_2IVO.pdb: