Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNHVASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTR-SDGIVALGGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQPDGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVE-SILEHAETLIEHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGY-GKVMHGEAVAMGMVQISKIAEEKGLMPAGITQSITEMCQKFGLPVDYEN-WEVDKLYQALTHDKKARGNTLKLVLVPELGSATIHPVSLEEMKDYLVK 5HVN Chain:A ((15-359)) --------SPSYNIIVDSVL--DFSHILEY-VTNKQVLVVTNTTVAKLYLTKFLAALVD-DLDVRTCILEDGEQYKSQQSLDKILSTLLENHFTRNSTVLVALGGGVIGDITGFAAAIYQRGIDFIQIPTTLLSQVDSSVGGKTAINHQLGKNMIGAFYQPKVVYTSIEFYKTLPQREYIAGMAEVVKYAFISK-DFYLWLDSNRDKILAKDSVTLIEMVKRSCQIKAQVVAMD---TGARAILNFGHTFGHAIEKCQNYRG-LKHGEAVGVGMAQAIDFSHYLGLISQQQAKDFNDFIVSFGISIDFPNDICQKEFLEAMLLD----NKELKFILIENIGSLSLQKQSKNELEQFLD-

General information: TITO was launched using: RESULT: Template: 5HVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1795 -80942 -45.09 -243.07 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -45.09 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_5HVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HVN-query.scw PDB file : Tito_Scwrl_5HVN.pdb: