Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEIEASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVN-ENGNLIGYNTDGKGFFKCL--PSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEKTRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVLPETLLVADIIYQPFETPFLKWARSQGNP-AVNGLGMLLYQAAEAFQLWTGKEMPTEEIWQSLTEKYQ
3SEF Chain:B ((33-294))----------AVFG---NHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLTERARLAGAVNTLKKLDDGEILGDNTDGEGLVQDLLAQQVLLKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQ---LAELVAAYG-EVKAQAFEQLKQ------SYDVIINSTSASLDGE-LPAIDPVIFSSRSVCYDMMYGKGYTVFNQWARQHGCAQAIDGLGMLVGQAAESFMLWRGLRPGTKQILREL-----


General information:
TITO was launched using:
RESULT:

Template: 3SEF.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1449 -55709 -38.45 -218.46
target 2D structure prediction score : 0.55
Monomeric hydrophicity matching model chain B : 0.76

3D Compatibility (PKB) : -38.45
2D Compatibility (Sec. Struct. Predict.) : 0.55
1D Compatibility (Hydrophobicity) : 0.76
QMean score : 0.418

(partial model without unconserved sides chains):
PDB file : Tito_3SEF.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-3SEF-query.scw
PDB file : Tito_Scwrl_3SEF.pdb: