Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLIVSVMPRSLEEAQA-LD-ATRYLDADIIEWRADYLPKEA----ILQVAPAIFEK-FAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQ-PDYIDFEYYSYKDVFEEML-----DFPNLVLSYHNFQETP--ENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNYTRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKIREILDEA 1QFE Chain:B ((16-252)) PKIIVSLMGRDINSVKAEALAYREA-TFDILEWRVDHFMDIASTQSVLTAARVI-RDAMPDIPLLFTFRSAKEGGEQTITTQHYLTLNRAAI-DSGLVDMIDLELFTGDADVKATVDYAHAHNVYVVMSNHDFHQTPSAEEMVSRLRKMQALGADIPKIAVMPQSKHDVLTLLTATLEMQQHYADRPVITMSMAKEGVISRLAGEVFGSAATFGAVKQASAPGQIAVNDLRSVLMILHNA

General information: TITO was launched using: RESULT: Template: 1QFE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1214 -3630 -2.99 -16.35 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -2.99 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.513

(partial model without unconserved sides chains): PDB file : Tito_1QFE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QFE-query.scw PDB file : Tito_Scwrl_1QFE.pdb: