Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGLAANMIGVQKRVIIFNLG-LVP-VVMFNPVLLSF-EGSYEAEEGCLSLVGVR-STKRYETIRLAYRDSKWQEQTITLTGFPAQICQHELDHLEGRII 5KOB Chain:B ((39-158)) -------------------------LVKDMFETM--HDANGAGLAAPQIGVNLQVVIFGFGPPVPETVLINPTITPVSQDMEEGWEGCLSVPGLRGAVSRFSMIKYHGFDQYGKPIDRVAEGFHARVVQHECDHLIGKL-

General information: TITO was launched using: RESULT: Template: 5KOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 503 -42825 -85.14 -396.53 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : -85.14 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_5KOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KOB-query.scw PDB file : Tito_Scwrl_5KOB.pdb: