Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLTQMPSEFQKA-LPVLEKIKEAGFEAYFVGGSVRDALLHSP---IHDVDIATSSYPEETKQIFPRTADIGIEHGTVL-------------VLDG----DEEYEVTTFRTEDVYVDYRRPSAVSFVRSLEEDLKRRDFTVNAFALDET---------GEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIESQAYDYLPDMASSQDKLNRLFDLE-TDFTF-----------------------------------ESSEQAWAALL-WALEIEN--------------AQSFLKSWKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQSLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLREKK 3WFP Chain:C ((2-362)) ------LNFYLSYFDDVAKVL-PREHYCFIVGGWVRDRILGEPVGYNIDVDFLTTADPVELAKNFAK--RIGGHFFVFEKRGFLIKRPTIASVVLHLPPYRYRFDFSPLKGK------------DLEKALIEDLKERDFTANAIAVNLDDVLSIGAKQTIVYDPTGGIKDLEQGLLRPVS--IENLKRDPVRVLRGFRIAIEKNLQLTEDFYEFVKEDPRIVLKSAVERITHELFKIMKEKTAHKVIRELYEYGVLEAIIPEIGRLREVKD----PLDEHTLKTLEYLEQVIEDRAKYLSAELLENFGKKRVLGEFTDVELLKWGALFHDIGKPQTFVTFYEHDKVGAQIVREIGERLRWGDEATEFVAKLVRHH---------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3WFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1218 37093 30.45 138.92 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 30.45 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.433

(partial model without unconserved sides chains): PDB file : Tito_3WFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3WFP-query.scw PDB file : Tito_Scwrl_3WFP.pdb: