Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKTILVTGGTSYIGSHTVKALL-NAGYQVHILDNL--STGNRAAVDSR---------------------ASFKELDVYDASALKA-YLEENQIDAVLHYTGEIVVSESIENPSKYFTANVAGMNQVLKVLSEVGIQKIMFSSTASLYGNNCID--KPVTEDTLLDPVNPYAETKLMGERMIYWMANRYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNI----------NKTALGQNDS----LKIFGDDYDTRDGSCIRDYIYVLDLA 1GY8 Chain:C ((5-279)) ----VLVCGGAGYIGSHFVRALLRDTNHSVVIVDSLVGTHGKSDHVETRENVARKLQQSDGPKPPWADRYAALEVGDVRNEDFLNGVFTRHGPIDAVVHMCAFLAVGESVRDPLKYYDNNVVGILRLLQAMLLHKCDKIIFSSSAAIFGNPTM-NAEPIDINAKKSPESPYGESKLIAERMIRDCAEAYGIKGICLRYFNACGAHEDGDIGEHYQGSTHLIPIILGRVMSDIAP-------DASTDKRMPIFGTDYPTPDGTCVRDYVHVCDLA

General information: TITO was launched using: RESULT: Template: 1GY8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1022 -38962 -38.12 -176.30 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -38.12 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.437

(partial model without unconserved sides chains): PDB file : Tito_1GY8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GY8-query.scw PDB file : Tito_Scwrl_1GY8.pdb: