Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MNTFVDAARSFRTQFDLNFSEKIIVDF----------FAGGGGASTGLEM-GLNRPVYVAV----NHNPKAISMHEA---NHPHAKHYIQDVFAVDPIDICDGHQVGWFHASP------DCTHHSQAAGGQPRKK-------EIRDLS-----WVVLKFA------GKVKPD----VISLENVKQILGWGPLIAKRDKATGRV------ITLDKININGKKVNRIAEPG----ERVPRHNQ----FLVPNPKKKGKTWKHFVRS--LEQLGYEVEWQKNIIAADFGAPTKRERLFLIARCDGQP---------IVWPE----KYFSKKPKG----NLKKWRS----TVECVDF-----SDLGNSIFDRPQGPLADATLKRIAKGIQKYVIETKEPFFVNSSTPFIGRDFRTSFGH-DIREPLATTTAGYGGHSSLISPILVPFITEFANASQQRNWSIDEPLSTICAQVKGGHHGLVTAKLSKDNYKGALRVAAFLINYYGNGDARSITEPMDTITTKDRLVLVTVWI----KGEPWAIVDICIRMLK--PRELFRAQGVPDSYVIEYGSDG--KPLSKKDQ--VFMV------GNSVSPYPMAAIARANNPFITQQIKGAA---- 5L8S Chain:A ((1-604)) MINFPKPTVEQFFRTYTITNFAVSSDEKRLVFNANLNGKMNLWAMDLPDTYPYLFAHRDESCNFIKFDPENRYVLAGFDKDGDENYQIYAIPNEGGLPHPLITGDASEKYYFSHLSA-DGKCV-YYETSKENPSFLNTRIRNLETGEDRLLNVGEVSTTELAAVSENEESFVYLRAFANTYIVGFVKMGEETFNITPDPEKVHVAMEPVFTDNETIYFATDYDSDEMYLAKFDLTSKEFSKVLAFDGESIQSVKWDKDNKAFYLITVKGVTDILYRYDVATDKVEECSLPVDIIEQIQVA------KSGNLYILGRSATVPHNVYQSSNGVEWKQLTNNRVLGLSPEDMVEPDIVSYTSFDGMEIEALLFKAKPENDNGYTIFWPHGGPQ----------SAERKMFRSMFQCFINRGYTIFAPNFRGSTGYGSAFTKLVELDWGEGPRLDCIAGIEWLFESGFTDRNK--------------LFLVGGSYGGYMALLLHGRHSDYFR---AVVDIFGPSDLFTFINSVP----PHWKPIMERWLGDPERDKERFIKDSPVTYLDGMVKPMLVIQGAKDPRVVKEESDQIVAKLKEKGRDVEYLVLEDEGHGFSKKENEIKVYSLMLAFLEKHQALEHHHHHH

General information: TITO was launched using: RESULT: Template: 5L8S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2722 71150 26.14 148.85 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 26.14 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.154

(partial model without unconserved sides chains): PDB file : Tito_5L8S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L8S-query.scw PDB file : Tito_Scwrl_5L8S.pdb: