Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIAALHQPSQVQLNQDGNLKHFLTIEGLSKENLTKILDTAQSFLDDNNNLINRPLLEGRTVMNLFFENSTRTRTTFEAAAKRLSANVLNIDIARSSTSKGETLRDTLWNLEAMAADIFVVRHSSSGAAHFIAKDVCPKVAIINAGDGRHAHPTQAMLDMLTIRREMKKPFEDLSVAIIGDIKHSRVARSDVAALQTLGCKDIRVIAPNTL-LPVGFSEY----GDHVRLFNNMDEGITGCDVIIALRIQNERIDSPALSSQSEFYRMYG---LNKERLSLAKPDCIVMHPGPMNRGVEIDSSIADGEQSVILKQVTNGIAVRMAVLALSMQGQLQEQGLIDAIAL 5G1O Chain:E ((13-308)) --------------------QHILSVQQFTKDQMSHLFNVAHTLRMMVQKERSLDILKGKVMASMFYEVSTRTSSSFAAAMARLGGAVLSFSE-------GESLADSVQTMSCY-ADVVVLRHPQPGAVELAAKH-C-RRPVINAGDGVGEHPTQALLDIFTIREELGT-VNGMTITMVGDLKHGRTVHS-LACLLTQYRVSLRYVAPPSLRMPPTVRAFVASRGTKQEEFESIEEALPDTDVLYMTRIQKER-----FG----YEACFGQFILTPHIMTRAKKKMVVMHPMP--RVNEISVEVDSDPRAAYFRQAENGMYIRMALLA------------------

General information: TITO was launched using: RESULT: Template: 5G1O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1413 -106462 -75.34 -384.34 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain E : 0.76 3D Compatibility (PKB) : -75.34 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_5G1O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G1O-query.scw PDB file : Tito_Scwrl_5G1O.pdb: