Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MATPFWYNTALHLLKPFYRWRIKRRAESLELYQQECLERFGPFEAPKNVKAIWFHAVSVGETNAAQPLIEYYLKLGQPVLVTNTTKTGQARAK--SLFLKEPYLDLFQAVYLPVDQ----------------KPLLKKFFEL--YQPKLLALVETELWPNLIDQAKLQHVPCLLLNARLSEKSAKGY--------------G--KVSGLTAGMLKQLDWVLAQDSATR--QRYVEL-----GL-DEHKSQVVGNIKFDIHAPEAFIKQ-----AAQLRQQWYLE---NRQVVTIASTHAP-EEQQILEAFAPYLNS--DRELVCIVVPRHPER-------------FDEVFEICQNLNLITHRRSMGQSIHASTQVYLADS--MGELWLWY----ALSQVCFVGGSLNEPGGGHNILEPMVLNVPTVVGPRYFNFQTIVDEFIDENAVLIAQDAQQVVDIWLACLAEPEATEQLVAQAHKVLQRNQG---SLQKHIGVINRYLAEKS 2R60 Chain:A ((32-461)) ---------------------------------------------------------FGGQLVYVKEVSLALAEMGVQVDIITRRIKDENWPEFSGEIDYYQETNKVRIVRIPFGGDKFLPKEELWPYLHEYVNKIINFYREEGKFPQVVTTHYGDGGLAGVLLKNIKGLPFTFTGHSLGAQKMEKLNVNTSNFKEMDERFKFHRRIIAERLTMSYADKIIVSTSQERFGQYSHDLYRGAVNVEDDDKFSVIPPGVNTRVFDGEYGDKIKAKITKYLERDLGSERMELPAIIASSRLDQKKNHYGLVEAYVQNKELQDKANLVLTLRGIENPFEDYSRAGQEEKEILGKIIELIDNNDC-------------RGKVSMFPLNSQQELAGCYAYLASKGSVFALTSFYE--PFGLAPVEAMASGLPAV-VTRNGGPAEILDG-GKYGVLVDPEDPEDIARGLLKAFESEETWSAYQEKGKQRVEERYTWQETARGYLEVIQEIAD---

General information: TITO was launched using: RESULT: Template: 2R60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1587 54438 34.30 154.21 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 34.30 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.430

(partial model without unconserved sides chains): PDB file : Tito_2R60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2R60-query.scw PDB file : Tito_Scwrl_2R60.pdb: