Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPLLQIAEPGQSSAPHQHRIAIGIDLGTTHSLAATVLSGKPKVLNDVQNRRLLPSIVHYG-DNTTHYGEEAKPFIIADPKNTIVSVKRFMGRS------KADIKFQHPYELVGSEKNEMPAFETRAG-RKT--PVEISAEILKQLKDRAEDSLQNPVNGAVITVPAYFDEAQRQATRYAAQLAGLNILRLLNEPTAAAVAYGLDQESNLATDRNYVIYDLGGGTFDVSILRFSQGVFEVLATGGHTALGGDDLDRLI----VKWAKKQLNIDVLSDEDYAV--FIVAARQAKEQLSTQDSVELKL------LEATLTLDRPTFESIIQVALDKTISVCKRVLRDAKLELTDIQNVVLVGGSTRSYAVQKAVREVF-AQEPLCTINPDEVVAIGASITANQLIGNSQDGSLLLDVTPLSLGLETMGGLVERLISRNTAIPVARRQEFTTYQDGQTAMLIHVVQGERDLVEHCRSLGRFVLHGIPPMTAGQARIEVTFQVDADGLLTVSAREATSGVQAHIDIKPSYGLSEADTERLLIEGFQHAEEDKNLRHLKETKVEAERELEALEQALKVDADLLDEKQLEALNSAKESLKAQLEGSDIQAIEQAVQQLKVHSDAFAALRMNRHIDHALKGTKLDDWSKSN 5EY4 Chain:B ((24-400)) ---------------------VGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKF-LPFKVV--EKKTKPYIQVDIGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREG---EKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKD-NRAVQKLRREVEKAKRALSSQHQARIEIESFYEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSG------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5EY4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1861 78427 42.14 222.80 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.71 3D Compatibility (PKB) : 42.14 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_5EY4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY4-query.scw PDB file : Tito_Scwrl_5EY4.pdb: