Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKLVVFSGAGMSAESGISTFRDSNGLWENYDIQQVATPEAWERNPALVQRFYNERRKNILEAQPNEAHQYIAKLQDHYDVQ-VITQNIDDPHERAGSQNVLHLHGNIRLAKSSGPDAQYTTQFYEVNGWKLDLEQDFCPNGYPL-RPHVVWFGEAVP--AYEEAIRLVQSADIFIVIGSTLSVYPVAALVHEIPHYSKAYYIAPQADHSRVPPQYKLLNMTATEGMHELFNQLTS 4BV2 Chain:B ((17-199)) ----VTLTGAGISTPSGIPDF------------QNVFDIDFFYSHPEEFYRFAKEGIFPMLQAKPNLAHVLLAKLEEKGLIEAVITQNIDRLHQRAGSKKVIELHGNVEEYYCVRCEKKYTVE--DVIKKLE---VPLCDDCNSLIRPNIVFFGENLPQDALREAIGLSSRASLMIVLGSSLVVYPAAEL----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4BV2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 773 -27998 -36.22 -169.68 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.72 3D Compatibility (PKB) : -36.22 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.403

(partial model without unconserved sides chains): PDB file : Tito_4BV2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4BV2-query.scw PDB file : Tito_Scwrl_4BV2.pdb: