Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTATALTLACSLLSVMSISQAQAADNIDVSFQTILQQERNWAGLQSKSLKVGDITWSYSEGGSSTKPTLLLIHGLAGSRDNWNRVAHYLTTNYHVIIPDLPGSGETIVSHDFDYSVPNLAEKLRRFVEAANLKGPIHIAGHSLGGSIALLYAGQYPFETKSLFLVDSGGIFRSANTIYLKDLTYLKQLLVSKKGDFNYLLKQTMFNP-PFIPREFLQAQEKLMINQAPQTQKLVDQLI-ALNKVYTPDSFAVLTKTIDAPTLILWGKQDKIINVEVANELKRLLKNAQPPVILENVGHMPILEAEQLVIQQYVPFLLKVETNQSSKTTTP 5EGN Chain:F ((4-261)) --------------------------------------------------VENDGVKIYYDSYG--EGVPIVFLHPFSTNGGIWYFQTFPFAQTNHVIVIDHRGHGRSDKPA-TGYSIMEHADDVVAVLDALK-VDRAVFVGNSIGGMIAMQLNLDHPQRVIGNLILSSGTGLGEGMPPEAGAAFQ-NDYIG----AFGGLLEGAVSARSKRERPEILAVMKAHFSVPSNFPKHVFDAATADPNGVFAW-NIKDRLSSIQAPTLVVAGEEDLVTTVANNQLLADNIPG-AELRVINDVGHFYQLERPSEFNELLRGFVA-------------

General information: TITO was launched using: RESULT: Template: 5EGN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 1503 -88829 -59.10 -346.99 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain F : 0.64 3D Compatibility (PKB) : -59.10 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_5EGN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EGN-query.scw PDB file : Tito_Scwrl_5EGN.pdb: