Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLDKKSVHR--KTFKQEELILIKKLDLNETTVAMPENLFQLL------IWTSD------------------LAQHNHALFIQNKNELELVGENDQNKKQELTDKINFYKKNALIENQTKDFYLNKLLNQGDLAVKGYIQHPTRTQTKYAIIPYQGYDFITFATREIIEK-FPIHFIDFKNEGAPQTLEDIHADTEELLSKVKDFVKSFREKYKEAVKKNKLIKEHNAAVH---EIYIKIKNGEAKLIKDVAEEVPRVEETAPIQTVNSNEKPIKVIKKRKFALVKNE 1JTD Chain:B ((1-273)) -VAATSVVAWGGNNDWGEATVPAEAQSGVDAIAGGYFHGLALKGGKVLGWGANLNGQLTMPAATQSGVDAIAAGNYHSLALKD-GEVIAWGGNEDGQTTVPAEARSGVDAIAAGAWASYALKDGKVIAWGD-DSDGQTTVPAEAQSGVTALDGGVYTALAVKNGGVIAWGDNYFGQTTVPAEAQSGVDDVAGGIFHSLALKDGKVIAWGDNRYKQTTVPTEALSGVSAIASGEWYSLALKNGKVIAWGSSRTAPSSVQS--GVSSIEAGPNAAYALKG---------

General information: TITO was launched using: RESULT: Template: 1JTD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1510 101497 67.22 417.68 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain B : 0.61 3D Compatibility (PKB) : 67.22 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.064

(partial model without unconserved sides chains): PDB file : Tito_1JTD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JTD-query.scw PDB file : Tito_Scwrl_1JTD.pdb: