Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEKGVETMKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKKYLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGALLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGSFYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLN----GVADVVVT-DGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEMDYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE 3TSN Chain:C ((277-314)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------YLPYPLVADTAFTKTGLKNCNRLVAMYHDLALAPLKAL-------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3TSN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 55 -9424 -171.35 -285.58 target 2D structure prediction score : 0.33 Monomeric hydrophicity matching model chain C : 0.52 3D Compatibility (PKB) : -171.35 2D Compatibility (Sec. Struct. Predict.) : 0.33 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.519

(partial model without unconserved sides chains): PDB file : Tito_3TSN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TSN-query.scw PDB file : Tito_Scwrl_3TSN.pdb: