Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRTRRLGQTLFSQPFLGEISMNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGTRDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEK-VGAEVVAITGSNGKTTTKDLTASVLSEKYK---TYKTQGNYNNN----------------------------I-------GLPYTILHM-PQDTEKIVLEMGMDHANEITELSLMAQPKAAAITMIGEAHIENLG-SREGIAKAKMEITDGLVSDGLLVVPADE-PL-LTPLTATLVQ------TVTTFGIGNGDVHAEVTAKGKAQTDFVVE-GETFTIPLPGSYNVTNALIAYTIGRF--FGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMGLVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQM------HALKEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK 3NRS Chain:A ((34-427)) ------------------------------------------------------------------------------------------------------GLERVKQVAERLDLLKPAPKIFTVAGTNGKGTTCCTLEAILLAAGLRVGVYSSPHLLRYTERVRIQGQELSEAEHSHSFAQIEAGRGDISLTYFEFGTLSALQLFKQAKLDVVILEVGLGGRLD---ATNIVDSDVAAITSIALDHTDWLGYDRESIGREKAGVFRGGK---PAVVGEPDMPQSIADVAAELGAQLYRRDVAWKFSQ--------------NGWHWQCGERQLTGLPVP-NVPLANAATALAVLHYSELPLSDEAIRQGLQAA-SLPGRFQVVSE-QP-LLILD-VAHNPHAARYLVNRLAQVI--GKVRAVVGMLS---D---KDIAGTLACLSER-VDEWYCAPLEGPRGASAGQLAEHLV------SARQFS-DVETAWRQAMQDADTQDVVIVCGSFHT-------------

General information: TITO was launched using: RESULT: Template: 3NRS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1754 -15786 -9.00 -50.27 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -9.00 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_3NRS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3NRS-query.scw PDB file : Tito_Scwrl_3NRS.pdb: