Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQ--KRQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFE--ASTSSVQKGETLYDTVLTMSALGVDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGLKVAIVGDITHSRVAKSNMQMLKRL-GAQVFFSGPR---------EWYDDEFEVYGHYMPLDELLDQVDVMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADSLVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA 1EKX Chain:B ((8-306)) ----------KHIISINDLSRDDLNLVLATAAKLK--ANPQPELLKHKVIAS-CFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTY-VDAIVMRHPQEGAA-RLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAMVGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEEVMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMK---VLHPLP--RVDEIATDVDKTPHAWYFQQAGNGIFARQALLALVLN---

General information: TITO was launched using: RESULT: Template: 1EKX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1429 34125 23.88 119.74 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 23.88 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.401

(partial model without unconserved sides chains): PDB file : Tito_1EKX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EKX-query.scw PDB file : Tito_Scwrl_1EKX.pdb: